以感染锈斑病的平菇为试材,采用组织分离法、柯赫法则、病原菌16SrDNA序列测定的方法分离并鉴定病原菌;采用测定细菌生物量的方法测定病原菌的适宜生长温度、酸碱度和盐度;采用抑菌圈法检测病原菌对抗生素敏感性,以期为研究病害发生与流行规律以及探索有效防治措施奠定基础。结果表明:锈斑病病原菌鉴定为托拉斯假单胞杆菌Pseudomonas tolaasii;该病原菌适生长的温度为21~30℃,适宜生长pH为7~8,适宜生长盐度为0.5%~2.5%;该病原菌对红霉素、氨苄青霉素不敏感;对链霉素、四环素、庆大霉素和土霉素的最低抑制浓度分别为312.0、156.0、78.0、78.0μg·mL~(-1)。 Pathogen was isolated and identified by tissue isolation method, Kohn’s law and 16S rDNA sequence of pathogen. The suitable growth temperature, pH value and salinity of pathogen were determined by measuring bacterial biomass. The antibacterial zone method was used to detect the sensitivity of pathogens to antibiotics, with a view to laying the foundation for studying the occurrence and prevalence of diseases and exploring effective prevention and control measures. The results showed that the pathogen of rust was identified as Pseudomonas tolaasii. The pathogen was suitable for growth at 21-30 ℃, pH 7.0-7.5 and salinity 0.5-2.5% Erythromycin and ampicillin. The minimum inhibitory concentrations against streptomycin, tetracycline, gentamicin and oxytetracycline were 312.0, 156.0, 78.0 and 78.0 μg · mL -1, respectively.