论文部分内容阅读
本研究采用免疫组化ＬＳＡＢ法及图象分析技术，用抗ＥＧＦＥ的单克隆抗体对８例正常喉粘膜、２５例喉粘膜不典型增生及５３例喉鳞癌组织进行标记及分析。结果发现，随着喉粘膜病变的加重，ＥＧＦ－Ｒ的染色强度ＰＵ值有逐渐增高的趋势，与正常喉粘膜（ＰＵ＝１３．３６±４．２６）相比，喉癌（原位癌ＰＵ＝２１．６２±１０．４２，进展期癌ＰＵ＝２６．６３±６．９２）及重度不典型增生（ＰＵ＝２３．６４±７．２８）组织中ＥＧＦ－Ｒ过度表达。研究结果提示，ＥＧＦ－Ｒ与喉癌的发生有一定的关系，重度不典型增生有高度的恶变潜能
In this study, 8 cases of normal laryngeal mucosa, 25 cases of laryngeal mucosa atypical hyperplasia and 53 cases of laryngeal squamous cell carcinoma were labeled and analyzed by immunohistochemical LSAB method and image analysis technique with anti-EGFE monoclonal antibody. The results showed that, with the increase of laryngeal mucosal lesions, the staining intensity of EGF-R PU value has a tendency to increase, compared with the normal laryngeal mucosa (PU = 13.36 ± 4.26), laryngeal cancer (carcinoma in situ PU = 21.62 ± 10.42, PU = 26.63 ± 6.92 in advanced stage and overexpression of EGF-R in severe atypical hyperplasia (PU = 23.64 ± 7.28). The results suggest that EGF-R and laryngeal cancer have a certain relationship, severe dysplasia with a high degree of malignant potential