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目的:研究利用同步辐射光栅X线相衬成像技术,在无对比剂的情况下,对离体裸鼠胃癌肺转移灶及其内部血管结构进行清晰成像。方法:通过裸鼠尾静脉注射中分化胃癌细胞株SGC-7901,建立血行转移模型,处死小鼠后,开胸取出两肺,均匀切割标本,经4%甲醛溶液(10%福尔马林溶液)固定备用。实验于上海同步辐射光源(Shanghai Synchrotron Radiation Facility,SSRF)X线成像线站BL13W1进行。常规切片作苏木精-伊红(hematoxylin eosin staining,HE)染色,并与光栅X线相衬成像图像进行对比。结果:在无对比剂的情况下,第三代同步辐射光栅X线相衬技术能对胃癌肺转移灶及血管结构进行清晰成像,显示血管直径达到15~30μm,近似低倍光学显微镜下的图像。结论:光栅X线相衬成像对显示裸鼠胃癌肺转移灶中的新生血管具有较高应用价值。
OBJECTIVE: To study the imaging of lung metastasis of gastric cancer and its internal vascular structure in nude mice without contrast by using the technique of synchrotron radiation X-ray phase contrast imaging. Methods: The model of hematogenous metastasis was established by differentiating gastric cancer cell line SGC-7901 from the tail vein of nude mice. After the mice were sacrificed, the two lungs were removed by thoracotomy. The specimens were homogenized and the cells were homogenized in 4% formalin solution (10% formalin solution ) Fixed spare. Experiments were performed on the Shanghai Synchrotron Radiation Facility (SSRF) X-ray imaging line station BL13W1. Conventional sections were stained with hematoxylin eosin (HE), and compared with the images of the grating X-ray phase contrast imaging. Results: In the absence of contrast agent, the third-generation synchrotron radiation X-ray phase contrast technique can clearly show the lung metastases and vascular structures of gastric cancer, showing that the diameter of blood vessels reaches 15 ~ 30μm, which is similar to that under low magnification optical microscope . Conclusion: Grating X - ray phase contrast imaging has a high value in displaying neovascularization in lung metastasis of gastric cancer in nude mice.