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目的 探讨康莱特注射液诱导人肝癌细胞凋亡的作用 ,为康莱特注射液治疗肝癌提供理论依据。方法 采用免疫组织化学及透射电镜技术观察康莱特注射液对肝癌细胞凋亡的影响。结果 肝癌细胞经康莱特注射液培养 2 4h后 ,其凋亡的百分率明显高于对照组和空白组 ,有显著性差异 (P <0 .0 5 ) ,且细胞凋亡在 5 0 %以上者占 44.12 % (15 /3 4 ) ,而对照组及空白组无 1例凋亡超过 5 0 % ;电镜下可见较为典型的细胞凋亡的形态学变化 ,细胞核固缩 ,染色质凝集 ,呈新月型紧贴于核膜周边 ,核碎裂 ,凋亡小体形成等。结论 康莱特注射液能诱导肝癌细胞发生凋亡 ;诱导癌细胞凋亡可能是康莱特注射液治疗作用的重要机制。
Objective To investigate the effect of Kanglaite injection on apoptosis of human hepatocellular carcinoma cells and provide a theoretical basis for Kanglaite injection in the treatment of liver cancer. Methods Immunohistochemistry and transmission electron microscopy were used to observe the effect of Kanglaite injection on hepatoma cell apoptosis. Results The percentage of apoptosis of hepatocellular carcinoma cells was significantly higher than that of the control and blank groups after 24 hours of Kanglaite injection. There was a significant difference (P < 0.05) and apoptosis was above 50%. 44.12% (15/34), but none of the control and blank group had apoptotic cells exceeding 50%; the typical morphology of apoptosis, nucleus pyknosis, and chromatin condensation were observed under electron microscope. The lunar type clings to the periphery of the nuclear membrane, the nuclear fragmentation, and the formation of apoptotic bodies. Conclusion Kanglaite injection can induce apoptosis of hepatocellular carcinoma cells; induction of apoptosis of cancer cells may be an important mechanism of Kanglaite injection.