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现已确认肿瘤坏死因子α(TNFα)的直接细胞毒作用是引起组织损害,但导致细胞死亡的细胞内变化仍不了解.文章研究了TNFα对正常实质细胞的细胞毒效应.选用Sprague-Dawley大鼠,用胶元酶灌注术取得肝细胞,离心后获得的肝细胞纯度大于90%,活细胞数大于85%.肝细胞用含胰岛素、L-谷氨酰胺、地塞米松等williams培养液培养,实验组在培养液中分别加入重组鼠TNFα、LPS、胰高血糖素或其
It has been confirmed that the direct cytotoxic effect of tumor necrosis factor alpha (TNFα) is caused by tissue damage, but the intracellular changes that lead to cell death are still not understood.This paper studied the cytotoxic effect of TNFα on normal parenchymal cells.Using Sprague-Dawley Rats were obtained by collagenase perfusion, the purity of hepatocytes obtained after centrifugation was more than 90% and the number of viable cells was more than 85% .Hepatocytes were cultured in williams medium containing insulin, L-glutamine and dexamethasone , The experimental group in the culture fluid were added recombinant murine TNFα, LPS, glucagon or its