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目的研究磁场、噪声、高温三因素复合作用对机体热应激蛋白-70(HSP70)表达的影响。方法采用三因素二水平正交方法[L8(27)]实验设计,用免疫组化ABC法测定大鼠肝脏、脾脏和脑组织的HSP70表达量。结果磁场对大鼠肝脏、脾脏和脑组织HSP70表达有影响(P<0.01或<0.05);高温对肝脏和脾脏组织中HSP70表达有影响(P<0.05)。结论磁场是影响HSP70表达的主因素,高温有一定的作用,磁场、高温、噪声三因素之间的交互作用不明显。
Objective To study the effect of three factors of magnetic field, noise and high temperature on the expression of heat stress protein-70 (HSP70). Methods The orthogonal design of three factors and two levels [L8 (27)] was designed and used to detect the expression of HSP70 in liver, spleen and brain of rats by immunohistochemical ABC method. Results The magnetic field had an effect on the expression of HSP70 in liver, spleen and brain of rats (P <0.01 or <0.05). High temperature had an effect on the expression of HSP70 in liver and spleen (P <0.05). Conclusion The magnetic field is the main factor affecting the expression of HSP70. High temperature has a certain effect. The interaction between magnetic field, high temperature and noise is not obvious.