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用本室建立的绿脓杆菌(PA)对鸡胚皮肤的体外粘附模型,研究了各种不同的预处理对 PA 粘附的影响。酸处理皮肤能增强 PA 的粘附作用,而过碘酸钠处理则能抑制 PA 的粘附,提示粘附受体中含有糖成份。4种单糖(D-半乳糖,D-甘露糖,L-岩藻糖和 N-乙酰葡萄糖胺)处理细菌对 PA 的粘附作用无明显影响,而唾液酸能显著地抑制 PA 的粘附,提示唾液酸可能是 PA 的粘附受体成份。神经氨酸酶和 PA-Lectin 的处理,对 PA 粘附无任何作用。从 Slime 菌株 S6中提取的 SPS 能增强 S36和 S51菌株对皮肤的粘附,其抗血清则能抑制 S36和 S51菌株的粘附。透射电镜观察显示 S 菌株体外糖毡包结构。以上结果表明,SPS 是一种重要的粘附物质。
The in vitro adhesion model of chick embryo skin with Pseudomonas aeruginosa (PA) established in this study was used to investigate the effect of various pretreatments on PA adhesion. Acid treatment of the skin can enhance the adhesion of PA, and sodium periodate treatment can inhibit the adhesion of PA, suggesting that the adhesion receptors contain sugar ingredients. Four kinds of monosaccharides (D-galactose, D-mannose, L-fucose and N-acetylglucosamine) had no obvious effect on the adhesion of PA, while sialic acid could significantly inhibit the adhesion of PA , Suggesting that sialic acid may be the adhesion receptor component of PA. Neuraminidase and PA-Lectin treatment, PA adhesion has no effect. The SPS extracted from Slime strain S6 enhanced the skin adhesion of S36 and S51 strains, while the antisera inhibited the adhesion of S36 and S51 strains. Transmission electron microscopy showed S strain in vitro glyph package structure. The above results show that SPS is an important adhesion material.