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目的探讨HLA-DRB1基因多态性与反复呼吸道感染(RRI)的遗传关联性。方法采用序列特异性引物-聚合酶链反应(SSP/PCR)技术对43例RRI小儿和41例健康小儿HLA-DRB1的19个等位基因进行分析,计算各个位点的基因分布频率和相对危险性RR值;并进行卡方检验,筛选有意义基因。结果RRI患儿HLA-DRB1*9和HLA-DRB1*12的分布频率明显高于健康儿童,HLA-DRB1*7的分布频率明显低于健康儿童,观察组与对照组比较P<0.05,差异有显著性,其它各位点差异无显著性。疾病组与正常组之间进行逐个等位基因的比较,以相对危险性RR值计算,HLA-DRB1*9的RR值为3.95,HLA-DRB1*12的RR值为3.16。HLA-DRB1*7的RR值为0.30。结论HLA-DRB1*9和HLA-DRB1*12可能是RRI小儿的易感基因。HLA-DRB1*7可能是RRI小儿的保护基因。
Objective To investigate the genetic association between HLA-DRB1 gene polymorphism and recurrent respiratory tract infection (RRI). Methods Twenty-nine alleles of HLA-DRB1 in 43 children with RRI and 41 healthy children were analyzed by sequence specific primer-polymerase chain reaction (SSP / PCR) to calculate the frequency and relative risk of HLA-DRB1 at each locus Sexual RR; and chi-square test, screening of meaningful genes. Results The distribution frequency of HLA-DRB1 * 9 and HLA-DRB1 * 12 in children with RRI was significantly higher than that in healthy children, the distribution frequency of HLA-DRB1 * 7 was significantly lower than that in healthy children, P <0.05 in observation group and control group, Significance, no significant difference in other sites. The allele-by-allele comparison between the disease group and the normal group was 3.95 for HLA-DRB1 * 9 and 3.16 for HLA-DRB1 * 12, based on the relative risk RR. The RR of HLA-DRB1 * 7 was 0.30. Conclusion HLA-DRB1 * 9 and HLA-DRB1 * 12 may be susceptible genes in children with RRI. HLA-DRB1 * 7 may be a protective gene in children with RRI.