作者在1976～1984年期间,对57份未经选择的由急性乙型肝炎转变为慢性HBsAg携带状态的病人进行了血清学追踪观察。用斑点杂交技术测定病人血清中的HBVDNA;用放射免疫分析或酶联免疫吸附法测定HBsAg;用放射免疫分析测定HBeAg、抗-HBe和抗-δ。 57份患者的首份血清中有46份(81％)HBV DNA阳性、9份阴性、2份随访中暂时转为阳性。在46例HBV DNA阳性的病人中19例随访期间HBV DNA和HBeAg均保持阳性,27例的HBV DNA从血清中清 From 1976 to 1984, the authors performed a serologic follow-up of 57 unselected patients with chronic HBsAg carrier status from acute hepatitis B. HBsAg was measured in sera of patients by dot blot hybridization; HBsAg was measured by radioimmunoassay or enzyme-linked immunosorbent assay; and HBeAg, anti-HBe and anti-delta were measured by radioimmunoassay. Forty-six (81%) of the 57 sera from the first sera were positive for HBV DNA, nine were negative and two were temporarily positive for follow-up. HBV DNA and HBeAg were positive in 19 of the 46 patients with positive HBV DNA during the follow-up period. HBV DNA from 27 patients