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研究抗小鼠胸腺基质细胞(MTSC)Pf18-3单抗识别分子的表达特性。方法：采用流式细胞(FACS)和激光扫描共焦显微镜(CLSM)分析TSC单抗Pf18-3染色特征。结果：FACS分析发现Pf18-3单抗识别分子可表达于胸腺、胎肝和骨髓等不同来源的基质细胞系和腹腔巨噬细胞表面。新鲜分离的胸腺细胞Pf18-3识别分子表达阴性，但ConA活化可诱导并上调其表达，随活化时间延长表达增高，且主要表达于CD4+CD8+和CD4+CD8-两细胞亚群。CLSM分析可见：荧光沿胸腺细胞边缘呈环状分布，活化后荧光明显增厚向胞浆延伸，胞核不着色。结论：Pf18-3单抗识别分子共表达于胸腺基质细胞及活化的胸腺细胞，并与CD4+CD8+和CD4+CD8-亚群胸腺细胞活化密切相关。“,”To identify the expression of the molecule recognized by Pf18-3 mAb (Pf18-3 molecule) on various cells. Meth-ods: The expression of pr18-3 molecule was assayed by flow cytometry and confocal laser scanning microscope . Result: The molecule recog-nized by Pf18-3 mAb expressed on TSC and other stromal cells. Whereas, fresh thymocytes were Pf18-3 negative. Interestingly, the expressionof Pf18-3 molecule was gradually up-regulated on thymocytes after activation by ConA. This molecule mainly expressed on CD4+ CD8+ andCD4+ CD8- cells. Under confocal laser scanning microscope, the staining of fluorescence showed as ring around the cell, it changed grsduallystronger and thicker with activation. Conclusion: This study indicated that the Pf18-3 molecule was co-expressive molecule of MTSC and acti-vated tlymocytes,it was concemed closely about the activation of CD4+ C D8+ and CD4+ CD8- cells.