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为明确香蕉中不同基因型内源条斑病毒(endogenous Banana streak virus,eBSV)的激活及其机制,根据eBSOLV-GD(endogenous Banana streak OL virus Guangdong)的结构设计引物,采用内源条斑病毒基因型分析方法及RT-PCR检测法对其基因型、表达和激活进行了研究。结果表明,只含A基因组的香蕉不含eBSOLV-GD;含B基因组的香蕉均含eBSOLV-GD。在所检测的含B基因组的香蕉中eBSOLV-GD可分为6种基因型,部分为感染型的eBSOLV-GD。粉蕉和大蕉中eBSOLVGD的片段Ⅱ和Ⅲ能表达,eBSOLV-GD经组织培养后可激活产生BSOLV-GD,引起香蕉发病,发病率为8.6%~18.0%,表明发病率的高低与香蕉的基因型有关,与eBSV基因型无关。
In order to clarify the activation and mechanism of different genotypes of endogenous Banana streak virus (eBSV) in banana, primers were designed according to the structure of eBSOLV-GD (endogenous Banana streak OL virus Guangdong) Type analysis and RT-PCR detection of its genotype, expression and activation were studied. The results showed that bananas containing only A genome did not contain eBSOLV-GD; Banana containing genome B contained eBSOLV-GD. EBSOLV-GD can be divided into six genotypes and partly infectious eBSOLV-GD in banana containing B genome. The fragments of eBSOLVGD in Fenuga and plantains can be expressed in segments II and III. The eBSOLV-GD was activated by tissue culture to produce BSOLV-GD, causing the incidence of banana, with the incidence of 8.6% -18.0%, indicating that the incidence of eBSOLV- Genotype, but not eBSV genotype.