论文部分内容阅读
目的比较分析ｒＩＬ－２刺激对人脐带血和成人外周血淋巴细胞亚群及功能的影响。方法利用流式细胞技术对脐带血和成人外周血淋巴细胞亚群进行检测，用ｔ检验做统计学分析。结果新鲜未刺激的脐带血淋巴细胞在发育和功能方面均比成人外周血幼稚；两者对ｒＩＬ－２刺激的反应明显不同，脐带血的变化主要表现为ＣＤ４＋和ＣＤ８＋细胞亚群的成熟和记忆功能增强，即由ＣＤ４５ＲＡ＋向ＣＤ４５ＲＯ＋细胞转化，而成人外周血淋巴细胞主要为ＣＤ３３－ＣＤ１６＋的ＮＫ细胞比例增加和ＣＤ４＋和ＣＤ８＋细胞亚群的ＣＤ２５＋细胞比例增加，而ＣＤ４５ＲＡ＋和ＣＤ４５ＲＯ＋细胞亚群无明显变化。结论人脐带血和成人外周血淋巴细胞无论在构成还是对ｒＩＬ－２刺激的反应均不同
Objective To compare the effects of rIL-2 stimulation on subsets and functions of human umbilical cord blood and adult peripheral blood lymphocytes. Methods Flow cytometry was used to detect umbilical cord blood and adult peripheral blood lymphocyte subsets, using t test to do statistical analysis. Results Fresh and unstimulated umbilical cord blood lymphocytes were immature than adult peripheral blood in both development and function. The response to rIL-2 stimulation was significantly different between the two groups. The changes of cord blood mainly manifested as maturation and memory of CD4 + and CD8 + subsets The enhanced function of CD45RA + cells transformed CD45RO + cells. However, the proportion of CD33 + CD16 + NK cells in adult peripheral blood lymphocytes was increased and the proportion of CD25 + cells in CD4 + and CD8 + subsets was increased. However, there was no significant change in CD45RA + and CD45RO + . Conclusions Human umbilical cord blood and adult peripheral blood lymphocytes respond differently both to composition and to rIL-2 stimulation