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目的:研究茄科茄属植物白英Solanum lyratum全草的化学成分,探讨白英中部分甾体类化合物的抗肿瘤作用。方法:采用柱色谱以及重结晶等方法进行分离纯化,根据理化性质及多种波谱方法鉴定化合物结构,并对3个甾体类化合物进行体外抑制人体肝癌细胞Hep G2试验。结果:确定了12个化合物的结构,分别为:薯蓣皂苷元(diosgenin,1),替告皂苷元(ti-gogenin,2),替告皂苷元酮(tigogenone,3),薯蓣皂苷元-3-O-α-L-吡喃鼠李糖基-(1→2)-β-D-吡喃葡萄醛酸甲酯(diosgenin 3-O-α-L-rhamnopyranosyl-(1→2)-β-D-glucuroniduronic acid methyl ester,4),4-甲基胆甾-7-烯-3β-醇(4-methyl-cholesta-7-ene-3β-ol,5),刺槐素-7-O-芸香糖苷(acacetin 7-O-rutinoside,6),1,5-二羟基-3-甲氧基-7-甲基-蒽醌(1,5-dihydroxy-3-methoxy-7-methyl-an-thraquinone,7),1,3,5-三羟基-7-甲基-蒽醌(1,3,5-trihydroxy-7-methyl-anthraquinone,8),大黄素甲醚-8-O-β-D-葡萄糖苷(phy-scion-8-O-β-D-glucopyranoside,9),大豆脑苷Ⅰ(soya-cerebrosideⅠ,10),异香草醛(isovanillin,11),阿魏酸二十二酯(docosylfer-ulate,12)。低浓度下化合物2~4对Hep G 2无明显抑制作用,随浓度增加,对其生长抑制率增强。结论:化合物2,3,5~12为首次从该植物中分离得到。化合物2~4对人体肝癌细胞Hep G2生长有一定的抑制作用。
OBJECTIVE: To study the chemical constituents of solanum lyratum from Solanum lycopersicum Solanum lycopersicum and explore the anti-tumor effect of some steroidal compounds in Baiying. Methods: The compounds were separated and purified by column chromatography and recrystallization. The structures of compounds were identified by physicochemical properties and spectroscopic methods. Three steroidal compounds were tested in Hep G2 cells in vitro. Results: The structures of 12 compounds were identified: diosgenin (1), ti-gogenin (2), tigogenone (3), diosgenin-3 -O-α-L-rhamnopyranosyl- (1 → 2) -β (1 → 2) -β-D-glucopyranosyl methyl ester -D-glucuroniduronic acid methyl ester, 4), 4-methyl-cholesta-7-ene-3β-ol, Acacetin 7-O-rutinoside (6), 1,5-dihydroxy-3-methoxy-7-methyl-an- thraquinone, 7), 1,3,5-trihydroxy-7-methyl-anthraquinone (8) Phy-scion-8-O-β-D-glucopyranoside (9), soya-cerebrosideⅠ (10), isovanillin (11) (docosylfer-ulate, 12). At low concentrations, compounds 2 ~ 4 showed no significant inhibitory effect on Hep G2, but increased with increasing concentration. Conclusion: Compounds 2,3,5 ~ 12 were isolated from this plant for the first time. Compounds 2 to 4 have certain inhibitory effects on the growth of Hep G2 cells.