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目的探讨基质金属蛋白酶3(MMP-3)及其抑制因子2(TIMP-2)在肺鳞癌中的表达及临床意义。方法采用免疫组织化学方法(SP法),分别检测96例肺鳞癌组织和20例正常支气管黏膜上皮组织中MMP-3及TIMP-2的蛋白表达。结果 MMP-3及TIMP-2在96例肺鳞癌组织中的阳性表达率(分别为81.3%、51.0%)明显高于正常支气管黏膜上皮组织(分别为25.0%、15.0%);与无淋巴结转移的肺鳞癌组织相比,伴淋巴结转移的肺鳞癌组织中MMP-3、TIMP-2的阳性表达率明显升高,且差异均有显著的统计学意义(P<0.01)。MMP-3的阳性表达随临床分期增加而有增高趋势,且差异有非常显著的统计学意义(P<0.01);TIMP-2在中晚期肺鳞癌组织(Ⅲ)的阳性表达率较早期肺鳞癌组织(Ⅰ+Ⅱ)有下降趋势,但差异无统计学意义(P>0.05),二者之间表达也无明显相关性(r=0.095,P>0.05)。与无淋巴结转移肺鳞癌组织比较,有淋巴结转移肺鳞癌组织中,MMP-3的表达增高,TIMP-2的表达明显下降,二者之间表达呈负相关(r=0.294,P<0.05)。结论 MMP-3与TIMP-2的过度表达与肺鳞癌的发展、淋巴结转移及临床TNM分期关系密切。MMP-3和TIMP-2在肺鳞癌组织中的表达情况,对预测肺鳞癌的浸润、转移,判断复发危险等方面具有重要意义,并有望成为判断肺鳞癌预后的生物学标志物。
Objective To investigate the expression and clinical significance of matrix metalloproteinase 3 (MMP-3) and its inhibitor 2 (TIMP-2) in squamous cell lung cancer. Methods Immunohistochemistry (SP method) was used to detect the protein expressions of MMP-3 and TIMP-2 in 96 cases of squamous cell carcinoma of the lung and 20 cases of normal bronchial epithelium. Results The positive rates of MMP-3 and TIMP-2 in 96 cases of lung squamous cell carcinoma were significantly higher than those in normal bronchial mucosa epithelium (25.0%, 15.0%, respectively) Metastasis of lung squamous cell carcinoma compared with lymph node metastasis of squamous cell carcinoma of the MMP-3, TIMP-2 positive expression rate was significantly increased, and the differences were statistically significant (P <0.01). The positive expression of MMP-3 increased with the clinical stage, and the difference was statistically significant (P <0.01). The positive expression rate of TIMP-2 in advanced lung squamous cell carcinoma (Ⅲ) There was no significant difference between the two groups (P> 0.05). There was no significant correlation between them (r = 0.095, P> 0.05). Compared with those without lymph node metastasis, the expression of MMP-3 and the expression of TIMP-2 in lung squamous cell carcinoma with lymph node metastasis were significantly higher than those without lymph node metastasis (r = 0.294, P <0.05) ). Conclusion The overexpression of MMP-3 and TIMP-2 is closely related to the development of lung squamous cell carcinoma, lymph node metastasis and TNM staging. The expression of MMP-3 and TIMP-2 in lung squamous cell carcinoma is of great significance in predicting the invasion and metastasis of lung squamous cell carcinoma and judging the risk of recurrence, and is expected to become a biomarker for prognosis of lung squamous cell carcinoma.