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目的 观察实验性Ⅰ型糖尿病恒河猴的组织病理学变化。方法 7只恒河猴分别静脉注射不同剂量的链脲佐菌素 (streptozotocin ,STZ) ,建立STZ性Ⅰ型糖尿病恒河猴模型 ,观察其心脏、肾脏、胰脏、脾脏等组织病理学变化。结果 动物胰岛数量明显减少 ,分布稀疏 ,残存胰岛萎缩 ,胰岛大小不等 ,成纤维细胞增生。肾小球增大 ,毛细血管壁增厚、僵硬 ,肾小管内膜细胞玻璃样变性 ,肾小球球囊内皮细胞增生。心肌变性、坏死、淤血 ,心肌细胞肥大 ,中、小血管壁增厚 ,血管壁纤维组织增加 ,冠状动脉内膜局部增厚 ,内皮细胞增生。脾脏小动脉硬化。结论 通过对STZ诱发的Ⅰ型糖尿病模型胰岛、肾脏和心脏等结构病理观察说明 ,该动物模型可用于糖尿病组织病理研究和药物疗效的评价。
Objective To observe the histopathological changes of experimental type 1 diabetic rhesus monkeys. Methods Seven Rhesus macaques were injected intravenously with streptozotocin (STZ) at different doses to establish STZ type 1 diabetic rhesus monkeys. The pathological changes of heart, kidney, pancreas and spleen were observed. Results The number of islets in animals decreased significantly, the distribution was sparse, the remains of islet atrophy, the size of islets and fibroblasts proliferated. Glomerular enlargement, capillary wall thickening, stiffness, tubular degeneration of renal tubular cells, glomerular balloon endothelial cell proliferation. Myocardial degeneration, necrosis, congestion, cardiomyocyte hypertrophy, medium and small blood vessel wall thickening, vascular wall fibrosis, coronary thickening of the intima, endothelial cell proliferation. Splenic arteriosclerosis. Conclusion The histopathological observation of STZ-induced type 1 diabetes mellitus, such as islets, kidneys and heart, shows that this animal model can be used for the study of histopathology and drug efficacy in diabetic patients.