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目的:研究川芎嗪(TMP)对甲型流感病毒H1N1感染的人肺腺癌上皮细胞(A549)中炎性细胞因子的影响。方法:培养A549细胞,甲型流感病毒H1N1感染A549后,分为细胞对照(N)组、H1V1感染(M)组、奥司他韦对照(D)组,TMP高剂量(S)组及TMP低剂量(J)组。采用RT-PCR和Western blotting法检测各组细胞中IL-1β、IL-10、INF-γ、TNF-αm RNA及蛋白表达。结果:与N组比较,M组IL-1β、IL-10、TNF-αm RNA表达显著升高,而INF-γm RNA表达显著降低(P<0.01);与M组比较,D、S、J组IL-1β、IL-10、TNF-αm RNA表达均明显降低(P<0.01)。M组IL-1β、IL-10、INF-γ和TNF-α蛋白表达较N组显著升高(P<0.05);与M组比较,S、J组IL-1β、IL-10、INF-γ和TNF-α均显著降低(P<0.05,P<0.01)。结论:TMP可抑制流感病毒感染后炎性细胞因子IL-1β、IL-10、INF-γ和TNF-αm RNA表达及蛋白分泌,减轻炎性反应,并恢复机体免疫功能的稳定和平衡。
Objective: To investigate the effect of ligustrazine (TMP) on inflammatory cytokines in human lung adenocarcinoma epithelial cells (A549) infected with influenza A virus H1N1. Methods A549 cells were cultured and infected with influenza A virus H1N1. The A549 cells were divided into four groups: control group (N), H1V1 infection group (M), oseltamivir control group (D), TMP high dose group (S) and TMP Low dose (J) group. The expressions of IL-1β, IL-10, INF-γ, TNF-αmRNA and protein in each group were detected by RT-PCR and Western blotting. Results: Compared with N group, the expression of IL-1β, IL-10 and TNF-αmRNA in M group was significantly increased and the expression of INF-γmRNA was significantly decreased in M group (P <0.01) The levels of IL-1β, IL-10 and TNF-αmRNA were significantly decreased (P <0.01). The levels of IL-1β, IL-10, INF-γ and TNF-α in M group were significantly higher than those in N group (P <0.05) γ and TNF-α were significantly lower (P <0.05, P <0.01). Conclusion: TMP can inhibit the expression of inflammatory cytokines IL-1β, IL-10, INF-γand TNF-αmRNA and the secretion of protein after influenza virus infection, relieve the inflammatory reaction and restore the stability and balance of immune function.