Development of Surface Plasmon Resonance-Based Biosensor Immunoassay for Sulfamethoxazole

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The illegal use of veterinary drugs has led to the occurrence of residues in food of animal origin.This is a hazard to people’s health.In this paper,a sensitive and fast assay was developed to determine sulfamethoxazole (SMOZ) in PBS with a homemade surface plasmon resonance (SPR)-based biosensor which had a single flow channel and two references.The derivative of SMOZ was covalently immobilized to a carboxymethyldextran modified gold film.A recently developed monoclonal antibody against sulfamethoxazole was used to develop an inhibitive immunobiosensor assay in which the antibody was mixed with SMOZ of different concentrations prepared with PBS to construct a standard curve with a low limit of detection(LOD).The effect of matrix in milk was also studied.Calculating of the apparent concentration showed the cross-reactivity with other sulfonamides. The illegal use of veterinary drugs has led to the occurrence of residues in food of animal origin. This is a hazard to people’s health. This paper, a sensitive and fast assay was developed to determine sulfamethoxazole (SMOZ) in PBS with a homemade surface plasmon resonance (SPR) -based biosensor which had a single flow channel and two references. The derivative of SMOZ was covalently immobilized to a carboxymethyldextran modified gold film. A recently developed monoclonal antibody against sulfamethoxazole was used to develop an inhibitive immunobiosensor assay in which the antibody was mixed with SMOZ of different concentrations prepared with PBS to construct a standard curve with a low limit of detection (LOD). The effect of matrix in milk was also studied. Calculating of the apparent concentration showed the cross-reactivity with other sulfonamides.
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