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用基因芯片分析肥厚性疤痕与正常皮肤成纤维细胞在静息及TGF beta3刺激下的表达谱的改变 .正常成纤维细胞在TGF beta3刺激前后的表达谱与肥厚性疤痕成纤维细胞在TGF beta3刺激前的表达谱正相关 (r=0 .76 0 37) ,刺激后 2种成纤维细胞的表达差异与肥厚性疤痕成纤维细胞TGF beta3刺激前后的表达谱差异正相关 (r =0 .82 6 76 2 ) .构成以上差异的基因有 6 2 0条 ,涉及细胞骨架蛋白、细胞周期蛋白、细胞因子、受体、转录因子及糖、蛋白质和核酸代谢的酶类 ;未发现胶原蛋白Ⅰ ,Ⅲ的表达水平的改变 .结果提示 :1.肥厚性疤痕成纤维细胞在静息状态下表现出与正常细胞受TGF beta3刺激后相似的反应 ,其可能处于一种应激状态 ;2 .TGF beta3刺激后 2种细胞的反应差异与肥厚性疤痕细胞的异常反应相关 ,TGF beta3的作用背景可能是肥厚性疤痕的分子病理学基础之一 ;3 .基因表达变化涉及细胞因子、细胞周期调控、凋亡等 ,其具备疾病遗传异质性的分子基础 ;4.胶原表达无明显上升 ,提示在肥厚性疤痕发病中 ,胶原纤维及细胞外基质堆积有复杂的分子生物学基础 .
The gene expression profiles of hypertrophic scar and normal skin fibroblasts were determined by resting and TGF beta 3 stimulation using gene chip.The expression profiles of normal fibroblasts before and after TGF beta 3 stimulation were compared with those of hypertrophic scar fibroblasts stimulated by TGF beta 3 (R = 0.76 0 37). The difference of expression of the two fibroblasts after stimulation was positively correlated with the expression profile of hypertrophic scar fibroblasts TGF beta 3 before and after stimulation (r = 0.82 6 76 2). There are 620 genes that make up the difference, involving cytoskeletal proteins, cyclin, cytokines, receptors, transcription factors and enzymes that metabolize sugar, protein and nucleic acids; no collagen I, III The results suggest that: 1. Hypertrophic scar fibroblasts in resting state showed normal cells stimulated by TGF beta 3 similar responses, which may be in a state of stress; 2. TGF beta 3 stimulation The difference of response of the latter two kinds of cells is related to the abnormal reaction of hypertrophic scar cells. The role of TGF-β3 may be one of the molecular pathological basis of hypertrophic scar.3. The gene expression changes involve Cytokines, cell cycle regulation and apoptosis, which have the molecular basis of genetic heterogeneity of the disease; 4. Collagen expression was not significantly increased, suggesting that hypertrophic scars in the pathogenesis of collagen fibers and extracellular matrix accumulation of complex molecular biology Fundamentals of learning.