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酯酶染色法近年来才用于免疫学领域。最早是Mueller(1975)以α—萘乙酸酯作底物,将小鼠淋巴结组织切片置弱酸性孵育液中作用一定时间,发现淋巴结付皮质区T淋巴细胞浆中具有酸性α—萘酚醋酸酯酶(Acidα—NaphthylAcetate Esterase简称ANAE)特殊形态(即淋巴细胞浆中呈红褐色点状的酯酶活性产物)。随后Ranki等人相
Esterase staining in recent years for the field of immunology. Mueller was the earliest Mueller (1975) α-naphthalene acetate as a substrate, the mouse lymph node tissue culture in weak acid solution for a certain period of time found in the lymph nodes to pay cortical T lymphocyte plasma with acidic α-naphthol acetate Esophageal (Acidα-NaphthylAcetate Esterase referred to as ANAE) special form (that is, the lymphocyte plasma red-brown point-like esterase activity). Ranki et al