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目的观察18-α甘草酸(18-α-GA)对CCl4引起的大鼠慢性肝损伤的治疗作用。方法大鼠皮下注射25%CCl4橄榄油2 mL/kg,每周2次,连续13周,给药组于第5周起分别腹腔注射18-α-GA 15、30、60 mg/kg,每日1次,连续至第13周。于末次给药24 h后,处死动物,取血分离血清或血浆,测ALT、AST、总蛋白、白蛋白、球蛋白、唾液酸、一氧化氮及透明质酸。取肝组织测定羟脯氨酸含量并作病理学检查。结果 18-α-GA能改善CCl4引起慢性肝损伤大鼠的肝功能,降低NO水平,减轻肝组织炎症活动度及纤维化程度。结论 18-α-GA对CCl4引起大鼠慢性肝损伤具有治疗效果。
Objective To observe the therapeutic effect of 18-α-glycyrrhizin (18-α-GA) on CCl4-induced chronic liver injury in rats. Methods Rats were subcutaneously injected with 2 mL / kg of 25% CCl4 olive oil twice a week for 13 weeks. The rats in the treatment group were injected intraperitoneally with 18-α-GA 15, 30 and 60 mg / kg Day 1, until the 13th week. After the last administration for 24 h, the animals were sacrificed and the blood or serum was separated for determination of ALT, AST, total protein, albumin, globulin, sialic acid, nitric oxide and hyaluronic acid. Liver tissue was taken for determination of hydroxyproline content and pathological examination. Results 18-α-GA can improve liver function, reduce the level of NO, and reduce the activity of liver inflammation and fibrosis in CCl4-induced chronic liver injury rats. Conclusion 18-α-GA has a therapeutic effect on CCl4-induced chronic liver injury in rats.