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Inhibition of RECl3 on Ni2O3-induced DNA breakage of human embryo lung cell (HEL) and reduced content of negative superoxidative ion (O2) in guinea alveolar macrophage (AM ) was observed by means of single cell gel electrophoresis assay (comet assay) and cytochrome C assay respectively. Incubated with 2×10 cell/ml human embryo lung cell for 1 h at 37℃, 20μg. ml-1 of Ni2O3 could obviously induce DNA strand breakage compared with the control (P< 0.01). Add 10 μg.ml-1 CeCl3 or 10μg RECl3 with 20 μg·ml-1 Ni2O3 simultaneously in to HEL culture, the DNA strand breakage caused by Ni2O3 reduction significantly. Culture with 4×10 cell/ ml AM for 1 h at 37℃, 10, 20 μg· ml-1 of Ni2O3 could distinctly increase·O2 content in AM compared with the control (P< 0.05). In the similar way, RECl3, CeCl3 or LaCl3 could evidently decrease·O2 content induced by Ni2O3 in AM (P<0.01), but both kinds of RE compounds can not suppress·O2 content in AM.
Inhibition of RECl3 on Ni2O3-induced DNA breakage of human embryo lung cell (HEL) and reduced content of negative superoxidative ion (O2) in guinea alveolar macrophage (AM) was observed by means of single cell gel electrophoresis assay (comet assay) and cytochrome C assay respectively. Incubated with 2 × 10 cells / ml human embryo lung cell for 1 h at 37 ℃, 20μg. Ml-1 of Ni2O3 could induce induce DNA strand breakage compared with the control (P <0.01). Add 10 μg. ml-1 CeCl3 or 10 μg RECl3 with 20 μg · ml-1 Ni2O3 in in HEL culture, the DNA strand breakage caused by Ni 2 O 3 reduction significantly. Culture with 4 × 10 cells / ml AM for 1 h at 37 ° C, 10, 20 (P <0.05). In the similar way, RECl3, CeCl3 or LaCl3 could evidently decrease · O2 content induced by Ni2O3 in AM (P <0.01) ), but both kinds of RE compounds can not suppress · O2 content in AM.