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以野生醋栗番茄(Solanum pimpinellifolium)LA722和栽培番茄(Solanum lycopersicum)9706为材料,结合发芽期大规模盐胁迫筛选方法和AB-QTL技术进行耐盐QTL定位和转育研究,建立了包括280个BC3S1单株的高代回交群体,利用26个CAPS、66个SSR和39个SRAP多态性标记构建了分子遗传图谱并进行了耐盐性QTL定位。利用发芽20、24、28d的发芽指数分别检测耐盐相关的QTL,确定发芽24d时的指数为检测QTL的最优指标,共检测出11个与耐盐相关的QTL,8个来自于野生醋栗番茄LA722。研究获得了58个极耐盐株系,对部分耐盐株系进行QTL qST-8-1和qST-9-2的基因型分析,其中230-1和230-3株系含QTLqST-8-1,228-4株系含主效QTL qST-9-2,231-6,210-7,226-1,226-6,226-7和204-7株系同时含有2个主效QTL,这些株系的农艺性状接近栽培番茄9706,可作为耐盐育种种质。
Solanum pimpinellifolium LA722 and Solanum lycopersicum 9706 were used to study the mapping and translocation of salt tolerant QTLs in combination with large-scale salt stress screening and AB-QTL techniques in germination stage. A total of 280 The backcross population of BC3S1 was constructed. The molecular genetic map was constructed with 26 CAPSs, 66 SSRs and 39 SRAP polymorphic markers and the salt tolerance QTLs were mapped. The QTLs related to salt tolerance were detected by germination index of 20, 24, and 28 days after germination respectively. The index of 24 days after germination was determined as the optimal index for QTL detection. Eleven QTLs related to salt tolerance were detected and 8 were from wild vinegar Chestnut tomato LA722. 58 extremely salt-tolerant lines were obtained and the genotypes of QTLs qST-8-1 and qST-9-2 were detected in some salt-tolerant lines. Among them, 230-1 and 230-3 lines contained QTLqST-8- The main QTLs QST-9-2, 231-6, 210-7, 226-1, 226-6, 226-7 and 204-7 in line 1,228-4 also contained two major-effect QTLs. The agronomic traits of these lines were similar to that of cultivated tomato 9706 Salt-tolerant breeding germplasm.