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目的:研究生长激素释放激素受体剪接变体1(SV1),一种生长激素释放激素(GHRH)受体的变体在子宫内膜异位症的表达模式。方法:在位和异位子宫内膜组织、腹膜B细胞采自有或没有子宫内膜异位症的妇女,正常卵巢组织采自没有子宫内膜异位症的妇女。分离异位子宫内膜基质干细胞(ESC)并加入GHRH培养,GHRH和SV1在样品组织的基因表达由RT-PCR检测,BrdU的摄入由特定的分析系统检测。结果:54份子宫内膜异位组织中有34份(63.0%)表达SV1 mRNA,显著高于在位内膜组织(4.3%)和正常卵巢组织(0.0%)。GHRH mRNA在异位子宫内膜组织的表达为22.2%(12/54),在位内膜组织中为25.9%,而在正常卵巢组织中为100.0%(14/14)和腹膜B细胞为81.3%(13/16)。GHRH能刺激表达SV1的ESC增加BrdU摄入。结论:GHRH和SV1在异位内膜表达谱不同于在位内膜,GHRH激活SV1并刺激细胞的增殖。
OBJECTIVE: To investigate the expression patterns of growth hormone releasing hormone receptor splice variant 1 (SV1), a variant of the growth hormone releasing hormone (GHRH) receptor, in endometriosis. Methods: Eutopic and ectopic endometrial tissues, peritoneal B cells were collected from women with or without endometriosis, and normal ovarian tissue from women without endometriosis. Ectopic endometrial stromal stem cells (ESCs) were isolated and cultured in GHRH. The gene expression of GHRH and SV1 in the sample tissues was detected by RT-PCR. The BrdU uptake was determined by a specific analytical system. Results: 34 (63.0%) of 54 endometriosis tissues expressed SV1 mRNA, which was significantly higher than that in eutopic endometrium (4.3%) and normal ovary tissue (0.0%). GHRH mRNA expression in ectopic endometrium was 22.2% (12/54), eutopic endometrium was 25.9%, whereas in normal ovarian tissue 100.0% (14/14) and peritoneal B cells were 81.3 % (13/16). GHRH stimulates SV1-expressing ESC to increase BrdU uptake. CONCLUSIONS: The expressions of GHRH and SV1 in ectopic endometrium are different from those in eutopic endometrium. GHRH activates SV1 and stimulates cell proliferation.