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给成年雄性大鼠注射黄体生成素释放激素(LHRH)或其高活性类似物(LHRH-A),显示明显的抗性腺作用,睾丸的LH和催乳素受体丧失,雄激素合成减少伴有睾丸及附属性器官萎缩,并抑制精子生成.LHRH的这种抗生育作用至少部分是由于内源性LH大量释放,继之性腺失敏感的结果.也有一些实验提示是由于LHRH对性腺具有直接抑制作用.为证明在体对性腺有直接作用的实验常常使用去垂体的动物.考虑到去垂体本身也会影响到性腺的机能,本文在完整动物使用LH抗血清,然后观察LHRH-A对睾丸促性腺激素受体的影响,以期在尽量减少激素紊乱的条件下鉴定LHRH-A直接性腺作用的重要性.使用体重200～225g的成年雄性大鼠,一部分静脉注射0.5ml LH抗血清(马抗牛LH的抗血清),另一部分注射等量正常马血清.两小时后,分别皮下注射不同剂量(3,10,30或100ng)的LHRH-A(〔D-丝(丁叔)~6,去甘-NH_2~(10)〕LHRH-EA),48小时后处死,做睾丸促性腺激素
Adult male rats injected with luteinizing hormone releasing hormone (LHRH) or its highly active analogue (LHRH-A) showed marked resistance to gonadal activity, loss of testicular LH and prolactin receptors, decreased androgen synthesis accompanied by testicular And accessory organs atrophy, and inhibit sperm production.LHRH this anti-fertility at least partly due to a large number of endogenous release of LH, followed by the results of gonadal sensitivity.Also some experiments suggest that LHRH has a direct inhibitory effect on the gonads. In order to prove that there is a direct role in the body gonadal experiments often use the pituitary animals.Due to the pituitary gland itself will also affect the function of the gonadal, LH antiserum in the complete animal use, and then observe LHRH-A testis gonadotropin Receptor in order to minimize the hormonal disturbance identified under the conditions of LHRH-A direct gonadal use adult male rats weighing 200 ~ 225g, part of the intravenous injection of 0.5ml LH antiserum (horse anti-bovine LH Antiserum), and the other part was injected with the same amount of normal horse serum. Two hours later, different doses (3, 10, 30 or 100 ng) of LHRH-A NH_2 ~ (10)] LHRH-EA), 4 After 8 hours sacrifice, do testosterone gonadotropin