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在pH 7.40和离子强度0.15 mol/L的模拟生理条件下,托拉塞米(TOR)对牛血清白蛋白(BSA)的内源荧光产生较强的猝灭作用.从吸收光谱的变化、温度对猝灭作用的影响及猝灭常数判断该猝灭作用是TOR与BSA形成基态配合物而导致的静态猝灭过程.文中计算了TOR与BSA的结合常数K、结合位点数n和相关的热力学函数.在最大猝灭波长342 nm处,荧光猝灭程度(?F)与托拉塞米的浓度成正比,线性范围和检出限分别为0.02～5.0μg/mL和6.3 ng/mL.本文还研究了适宜的反应条件,考察了共存物质的影响,表明方法具有较好的选择性,据此提出了以BSA为探针快速测定痕量TOR的荧光光谱新方法,适用于服用TOR后尿样和片剂中托拉塞米的测定.
Under the physiological conditions of pH 7.40 and ionic strength of 0.15 mol / L, TOR exerted a strong quenching effect on endogenous fluorescence of bovine serum albumin (BSA). From the changes of absorption spectra, temperature Quenching Effect and Quenching Constant Judgment The quenching effect is a static quenching process caused by the formation of a ground state complex between TOR and BSA.The binding constants K of TOR and BSA, the number of binding sites n and the related thermodynamics The fluorescence quenching (? F) was proportional to the concentration of torsemide at a wavelength of 342 nm, with a linear range and a detection limit of 0.02 to 5.0 μg / mL and 6.3 ng / mL, respectively. The suitable reaction conditions were also investigated. The influence of the coexisting substances was investigated, indicating that the method has good selectivity. Based on this, a new fluorescence spectrometry method for the rapid determination of trace TOR with BSA was proposed. Determination of torsemide in tablets and tablets.