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目的探讨miR-200家族成员在乳腺癌中的表达及其临床意义。方法实时定量PCR和原位杂交分析乳腺癌组织中miR-200家族5个成员的表达情况。单因素和多因素分析miR-200a、miR-200b和miR-200c表达与乳腺癌临床病理参数和预后之间的相关性。结果 miR-200a、miR-200b和miR-200c在乳腺癌组织中的表达明显低于正常乳腺上皮组织,差异有统计学意义(P<0.01)。miR-200a和miR-200b低表达与乳腺癌分期更晚、分级更高有关,而miR-200c低表达仅与分期更晚有关。多元回归分析表明,miR-200a、miR-200b和miR-200c低表达与乳腺癌患者生存期更短有关(P<0.01),miR-200a、miR-200b和miR-200c是乳腺癌患者预后的独立预测因子。结论 miR-200a、miR-200b和miR-200c表达下调能够促进肿瘤进展,是预测乳腺癌患者预后的可靠指标,miR-200a、miR-200b和miR-200c也具有成为乳腺癌靶向治疗靶点的潜力。
Objective To investigate the expression of miR-200 family member in breast cancer and its clinical significance. Methods Real-time quantitative PCR and in situ hybridization were used to analyze the expression of miR-200 family members in breast cancer tissues. Univariate and multivariate analysis of miR-200a, miR-200b and miR-200c expression and clinicopathological parameters and prognosis of breast cancer correlation. Results The expression of miR-200a, miR-200b and miR-200c in breast cancer tissues was significantly lower than that in normal breast epithelial tissues (P <0.01). Low expression of miR-200a and miR-200b is associated with later stage and higher grade of breast cancer, whereas low expression of miR-200c is only associated with later stage of breast cancer. Multivariate regression analysis showed that the low expression of miR-200a, miR-200b and miR-200c correlated with the shorter survival of patients with breast cancer (P <0.01). The prognosis of breast cancer patients with miR-200a, miR-200b and miR- Independent predictors. Conclusions The downregulation of miR-200a, miR-200b and miR-200c can promote tumor progression, which is a reliable indicator of prognosis in patients with breast cancer. MiR-200a, miR-200b and miR-200c also have the potential to become target targets for breast cancer potential.