目的　观察黄连解毒汤有效部位对培养神经细胞谷氨酸兴奋毒性的保护作用。方法　体外培养新生大鼠皮层神经细胞 ,加入谷氨酸观察谷氨酸对神经细胞的兴奋毒性及黄连解毒汤有效部位的保护作用。结果　黄连解毒汤有效部位 30 5mg kg能明显增高模型细胞的活性 ;6 10、30 5、15 3mg kg能显著降低模型细胞乳酸脱氢酶 (LDH)漏出率 ,降低MDA生成 ;30 5、15 3mg kg可减少NO生成 ,6 10mg kg还可提高SOD活性。结论　黄连解毒汤有效部位对培养神经细胞谷氨酸兴奋毒性具有显著的保护作用 ,其机制可能与降低NO含量、抗过氧化作用有关。 Objective To observe the protective effect of active fraction of Huanglian Jiedu Decoction on glutamate excitatory toxicity of cultured neurons. Methods Rat cortical neurons were cultured in vitro. Glutamate was added to observe the excitotoxicity of glutamate on nerve cells and the protective effect of Huanglian Jiedu Decoction. Results The active fraction of Huanglianjiedu Decoction at 30 5mg kg can significantly increase the activity of model cells; 6 10, 30 5, 15 3mg kg can significantly reduce the leakage rate of lactate dehydrogenase (LDH) and reduce MDA production in model cells; 30 5, 15 3mg Kg reduced NO production, and 6 10 mg kg also increased SOD activity. Conclusion The effective fraction of Huanglian Jiedu Decoction has a significant protective effect on glutamate excitatory toxicity of cultured neurons, and its mechanism may be related to reducing NO content and anti-peroxidation.