细胞增殖通常用~3H-Tdr掺入法或活细胞计数法进行测定,前者由于使用放射性同位素易污染环境并且需要昂贵的仪器,后者则易受主观因素影响误差较大,因此两者的应用都有一定局限性。最近我们根据Mosmann介绍的MTT[3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide]比色分析法测定了小鼠EAC、L_(1210)、ARS等 Cell proliferation is usually measured by the ~3H-Tdr incorporation method or live cell counting method. The former uses radioactive isotopes to contaminate the environment and requires expensive instruments, while the latter is subject to subjective factors and has a large error. All have some limitations. Recently, we determined the mouse EAC, L_(1210), ARS, etc. using the MTT [3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide] colorimetric assay introduced by Mosmann.