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本文采用二甲氨基偶氮苯(DAB)诱发的大鼠肝癌模型,运用组织病理学、血清学、免疫组化及免疫电镜技术相结合的方法,对诱癌过程中各个不同时期肝脏的组织病理学变化及肝癌阳性标志物AFP的表达进行了动态观察。结果显示:早在血清AFP浓度上升和由卵圆细胞转变而来的小肝细胞表达AFP之前,肝小叶中就出现了散在的AFP阳性肝细胞,我们认为这种AFP阳性肝细胞可作为肝细胞癌前病变的早期征象之一。在AFP阳性的肝细胞内,AFP主要定位于核周间隙、粗面内质网和高尔基复合体。
In this article, a rat model of liver cancer induced by dimethylaminoazobenzene (DAB) was used. Histopathology, serology, immunohistochemistry, and immunoelectron microscopy techniques were used to determine the pathological changes of the liver during various stages of the cancer induction process. The dynamic changes of the histopathological changes and the expression of AFP, a positive marker of liver cancer, were dynamically observed. The results showed that scattered AFP positive hepatocytes appeared in the hepatic lobule as early as the increase in serum AFP concentration and the expression of AFP in small hepatocytes transformed from oval cells. We believe that this type of AFP positive hepatocytes can serve as hepatocytes. One of the early signs of precancerous lesions. In AFP-positive hepatocytes, AFP is mainly located in the perinuclear space, the rough endoplasmic reticulum, and the Golgi complex.