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目的:应用高效液相色谱法测定炎可宁片中大黄素,大黄酚的含量。方法:采用Shim-pack-C18柱(5μm,150×4.6mm),以甲醇-0.1%磷酸(85∶15)为流动相,检测波长254 nm。结果:大黄素、大黄酚线性范围分别为(0.016 128~0.080 64μg,r=0.999 7;0.032 48~0.162 4μg,r=0.999 8大黄素,大黄酚的回收率分别为97.88%,RSD=1.64%;97.93%,RDS=0.71%。结论:该方法用于测定炎可宁片中大黄素、大黄酚的含量,具有简便、准确、灵敏的特点,可作为该制剂质量控制的制标。
Objective: To determine the content of emodin and chrysophanol in Yankengning Tablets by HPLC. METHODS: A Shim-pack-C18 column (5 μm, 150×4.6 mm) was used with methanol-0.1% phosphoric acid (85:15) as the mobile phase. The detection wavelength was 254 nm. Results: The linear ranges of emodin and chrysophanol were (0.016 128-0.080 64 μg, r=0.999 7; 0.032 48-0.162 4 μg, r=0.999 8 emodin, respectively. The recovery of chrysophanol was 97.88% and RSD was 1.64%, respectively. 97.93%, RDS=0.71%.Conclusion: This method is used to determine the content of emodin and chrysophanol in Yankengning tablet, which is simple, accurate, and sensitive, and can be used as a standard for the quality control of the preparation.