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目的探讨凋亡相关基因caspase-3和bcl-2在大肠腺瘤及大肠腺癌组织中的表达及其意义。方法采用高通量的组织芯片技术及免疫组织化学S-P法检测115例大肠腺癌组织、19例大肠腺瘤组织和12例正常大肠组织中caspase-3和bcl-2的表达。结果在正常大肠黏膜、腺瘤和大肠腺癌组织中caspase-3的阳性表达率分别为83.33%、47.37%、41.74%,bcl-2的阳性表达率分别为25%、84.21%、58.26%;正常黏膜组织caspase-3的表达高于大肠腺瘤和大肠腺癌,而bcl-2表达低于后两者,差异均有统计学意义(均P<0.05)。caspase-3和bcl-2的阳性表达率与大肠腺癌患者的组织分化程度相关(P<0.05)。结论组织芯片技术是大规模平行检测多基因蛋白表达的一种有效方法。caspase-3和bcl-2可能在大肠腺癌的发生、发展中起了重要作用。
Objective To investigate the expression and significance of apoptosis-related genes caspase-3 and bcl-2 in colorectal adenoma and colorectal adenocarcinoma. Methods The expressions of caspase-3 and bcl-2 in 115 cases of colorectal adenocarcinoma, 19 cases of colorectal adenoma and 12 cases of normal colorectal tissues were detected by high-throughput tissue microarray and immunohistochemical S-P method. Results The positive rates of caspase - 3 in normal colorectal mucosa, adenoma and adenocarcinoma were 83.33%, 47.37% and 41.74%, respectively. The positive rates of bcl - 2 were 25%, 84.21% and 58.26%, respectively. The expression of caspase-3 in normal mucosa was higher than that in colorectal adenoma and colorectal adenocarcinoma, while the expression of bcl-2 was lower than the latter two (all P <0.05). The positive rates of caspase-3 and bcl-2 were correlated with the histological differentiation of patients with colorectal adenocarcinoma (P <0.05). Conclusion Tissue microarray technology is an effective method for large-scale parallel detection of multi-gene protein expression. Caspase-3 and bcl-2 may play an important role in the occurrence and development of colorectal adenocarcinoma.