论文部分内容阅读
目的:探讨妊娠中晚期不同CO2气腹时间对大鼠HPL含量的影响。方法:建立妊娠中期CO2气腹的大鼠模型,分实验组及对照组,采用固相夹心法酶联免疫吸附实验(ELISA)检测妊娠中晚期大鼠HPL的含量变化。结果:实验组妊娠中期大鼠HPL值与对照组相比,无统计学意义(P>0.05);妊娠晚期,常气腹压1 h组与2 h组(B、C组)HPL值与非气腹组(A组)相比,无统计学意义(P>0.05);高气腹压1 h组与2 h组(D、E组)HPL值明显低于同期非气腹组(A组),有统计学意义(P<0.01);高气腹压1 h组与2 h组HPL值低于常气腹压1 h组与2 h组,有统计学意义(P<0.05)。结论:不同孕期母鼠血液中HPL值不同,与胎盘成熟度呈正相关;不同气腹压力及时间对妊娠中期母鼠胎盘功能无明显影响,但对妊娠晚期母鼠胎盘功能有明显影响,压力越大、时间越长,影响越明显。
Objective: To investigate the effect of different CO2 pneumoperitoneum time on the content of HPL in rats during the second trimester of pregnancy. Methods: The model of CO2 pneumoperitoneum was established in the second trimester of pregnancy. The experimental group and the control group were divided into two groups. The content of HPL in the second and third trimester of pregnancy was detected by solid-phase sandwich enzyme-linked immunosorbent assay (ELISA). Results: Compared with the control group, the HPL value in the second trimester of pregnancy in experimental group was not significantly different (P> 0.05). In the third trimester of pregnancy, (P> 0.05). The HPL of high-pressure abdominal hyperbaric group in 1 h group and 2 h group (D, E group) was significantly lower than that in non-pneumoperitoneum group (group A) ), With statistical significance (P <0.01). The HPL of high-pressure abdominal pressure in 1 h group and 2 h group was lower than that in normal air pressure 1 h group and 2 h group (P <0.05). Conclusion: There is a positive correlation between HPL value and placental maturation in different pregnant mothers. Different pneumoperitoneum pressure and time have no significant effect on placental function in second trimester, but have significant effect on placental function in third trimester. Big, the longer, the more obvious the impact.