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目的探讨肝炎病毒的检测方法,评价酒精性肝病与肝炎病毒感染的关系。方法选择我院2008-2009年收治的150例酒精性肝病患者,采用Tecan酶标仪和ABI7000PCR扩增仪检测HBV和HCV感染情况,对其实验室检测结果进行分析结果。结果单纯酒精性肝病患者、酒精性肝病合并HCV感染者、酒精性肝病合并HBV感染者的饮酒量差异无统计学意义,共发生肝硬化为37例(占22.0%),各组肝硬化发病率相比差异有统计学意义。结论酒精对肝炎病毒复制和肝脏损害可能具有促进作用,建议将PCR技术与ELISA技术联合起来,用于肝炎病毒的检测。
Objective To investigate the detection of hepatitis virus and evaluate the relationship between alcoholic liver disease and hepatitis virus infection. Methods 150 patients with alcoholic liver disease admitted in our hospital from 2008 to 2009 were enrolled in the study. The detection of HBV and HCV infection by Tecan microplate reader and ABI7000PCR instrument was used to analyze the laboratory test results. Results There was no significant difference in alcohol consumption among patients with alcohol-induced liver disease and alcohol-related liver disease and with HCV infection. There were 37 cases of cirrhosis (22.0%) with alcoholic liver disease and HBV infection. The incidence of cirrhosis Compared with the difference was statistically significant. Conclusion Alcohol may promote hepatitis virus replication and liver damage. It is suggested that PCR and ELISA should be combined for the detection of hepatitis virus.