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目的研究葡萄籽提取物(GSE)对砷诱导肝损伤大鼠抗氧化防御系统和NADPH氧化酶的影响。方法40只健康雄性SD大鼠随机分为4组:正常对照组、砷暴露组(As,NaAsO230 mg/L)、GSE干预组(GSE100 mg/kg隔日灌胃)、砷暴露+GSE干预组(As+GSE)。12个月后,检测谷丙转氨酶(ALT)、谷草转氨酶(AST)和白蛋白,作肝功能分析;检测谷胱甘肽(GSH)、氧化型谷胱甘肽(GSSG)、超氧化物歧化酶(SOD)血清抗氧化酶的活性、检测组织活性氧(ROS)水平、检测脂质过氧化水平;Western blot检测肝脏中烟酰胺腺嘌呤二核苷酸磷酸(NADPH)氧化酶亚单位Nox2、Nox4、p47phox蛋白的表达。结果与对照组相比,砷干预组的肝功能明显受损,抗氧化酶活性降低,组织ROS水平、脂质过氧化水平、Nox2、Nox4、p47phox蛋白表达水平升高(P<0.01);与As组相比,联合干预组的各项指标均得到明显改善(P<0.01)。结论 GSE可抑制慢性砷暴露引起大鼠的氧化应激及肝损伤,其机制可能与抑制NADPH氧化酶活性有关。[营养学报,2012,34(4):388-391]
Objective To study the effects of grape seed extract (GSE) on antioxidant defense system and NADPH oxidase in arsenic-induced liver injury rats. Methods Forty healthy male Sprague Dawley rats were randomly divided into four groups: normal control group, arsenic exposure group (As, NaAsO230 mg / L), GSE intervention group (GSE100 mg / kg intragastric administration), arsenic exposure + GSE intervention group As + GSE). Twelve months later, ALT, AST and albumin were detected for hepatic function analysis; glutathione (GSH), glutathione (GSSG), superoxide dismutase (SOD) activity in serum were measured. ROS levels were measured and the levels of lipid peroxidation were measured. Western blot was used to detect Nox2, NADPH, Nox4, p47phox protein expression. Results Compared with the control group, the liver function of arsenic - treated group was significantly impaired and the activities of antioxidant enzymes decreased. The levels of ROS, lipid peroxidation and the expression of Nox2, Nox4 and p47phox proteins were increased (P <0.01) As compared with As group, all the indexes in combination intervention group were significantly improved (P <0.01). Conclusion GSE can inhibit oxidative stress and hepatic injury induced by chronic arsenic exposure in rats. The mechanism may be related to the inhibition of NADPH oxidase activity. [Journal of Nutrition, 2012,34 (4): 388-391]