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以水稻广亲和中粳品系02428为材料,分析了其胚性悬浮细胞系建立过程中氨基酸、多胺及内源激素的变化。结果表明:1.游离氨基酸的总量是FP_(15)>MP_(45)>LP_(90),其中FP_(15)游离精氨酸的含量很高,而在MP_(45)和LP_(90)中含量剧降。蛋白质氨基酸和蛋白质合成能力正好和游离氨基酸的总量变化相反。2.从FP_(15)→MP_(45)→LP_(90),腐胺含量呈上升、精胺含量呈下降趋势。腐胺/(精胺+亚精胺)上升了近20倍。悬浮细胞经脱壁而成游离原生质体后,其总多胺、尸胺和腐胺的含量与悬浮细胞相比均大幅度下降,而精胺和亚精胺则有所升高。3.植物内源激素IAA、2ip的含量是LP_(90)>MP_(45)>FP_(15),ABA、ZRs的含量是FP_(15)>MP_(45)>LP_(90)。上述变化在一定程度上解释了胚性悬浮细胞即后期悬浮细胞适合于进行原生质体培养的生理生化机理。
With the medium and high japonica rice line 02428 as materials, the changes of amino acids, polyamines and endogenous hormones during the establishment of embryogenic suspension cell lines were analyzed. The results showed that: 1. The total amount of free amino acids was FP_ (15)> MP_ (45)> LP_ (90), in which FP_ (15) ) Content in the sharp decline. Protein amino acids and protein synthesis capacity and free amino acid changes in the total amount of the opposite. From FP_ (15) → MP_ (45) → LP_ (90), the content of putrescine increased and the content of spermine decreased. Putrescine / (spermine + spermidine) rose nearly 20-fold. After the detached cells were detached from the free protoplasts, the content of total polyamines, cadaverine and putrescine decreased significantly compared with the suspension cells, but the spermine and spermidine increased. Plant endogenous hormones IAA, 2ip content is LP_ (90)> MP_ (45)> FP_ (15), ABA, ZRs content is FP_ (15)> MP_ (45)> LP_ (90). To some extent, these changes explain the physiological and biochemical mechanisms that embryonic suspension cells, late-stage suspension cells, are suitable for protoplast culture.