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在被测交的5个甘蓝型油菜波里马细胞质雄性不育恢复系中,没有1个能使显性细胞核雄性不育系恢复可育性。分别以纯合型显性细胞核雄性不育系Rs1046和显性细胞核雄性不育杂种中杂3号作父本与具有不育细胞质的波里马细胞质雄性不育恢复系花叶恢杂交,成功地将显性细胞核雄性不育基因导入到波里马细胞质雄性不育恢复系花叶恢中。以作者自己选育的具有不同来源的双低甘蓝型油菜波里马细胞质雄性不育恢复系作父本与得到的具有波里马细胞质的显性细胞核雄性不育株不断回交,并将所有回交后代种子混合种植,建立起了一个甘蓝型油菜波里马细胞质雄性不育恢复系的轮回选择群体。
None of the five male-sterile restorer lines of Pollock in test-fertile Brassica napus could restore the dominant male sterile line to fertility. Respectively, with homozygous dominant male sterile line Rs1046 and dominant male sterile hybrid Zhongza3 as the male parent sterile cytoplasmic male sterile line with Cymbidium cytoplasmic male sterile restore, will be successfully The dominant-negative nuclear male sterility gene was introduced into the pollen cytoplasm male sterile restorer line. The self-bred double-loving Brassica napus rape with different origins Polymorphic Cytoplasmic Male Sterile Restorer lines were parent and obtained with Polima cytoplasm of the dominant male sterile line of nuclear continuously backcrossed and all Backcross hybrids were planted in the backcross generation, and a cycle selection group was established for the restorer line of the Polymorphic male sterile line of Brassica napus.