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目的 :ＣＲＯＣ - 1是人细胞中新发现的基因 ,其酵母同源基因ＭＭＳ2是一个抗增变基因。本研究用反义策略建立ＣＲＯＣ - 1基因表达阻断细胞系 ,分析ＣＲＯＣ - 1基因在细胞生理活动中的作用。方法 :1 ＣＲＯＣ - 1反义ＲＮＡ表达质粒构建 :用ＡＣＣＩ酶从ｐＢＳ -Ｃｒｏｃ- 1
Objective: CROC - 1 is a newly discovered gene in human cells. Its yeast homologous gene MMS2 is an anti - mutation gene. In this study, CROC - 1 gene expression blocking cell line was constructed by antisense strategy and the role of CROC - 1 gene in cellular physiology was analyzed. Methods: 1 CROC - 1 antisense RNA expression plasmid construction: ACCI enzyme from pBS-Croc-1