论文部分内容阅读
目的探讨壮族人群单核细胞趋化蛋白1(MCP-1)启动子区A—2518G基因多态性与血清中MCP-1含量与糖尿病(DM)及糖尿病肾病(DN)的关系。方法采用聚合酶链反应-限制性片段长度多态性方法,对1 50例T2DM患者[正常白蛋白尿(D_0)组51例,微量白蛋白尿(D_1)组54例,临床白蛋白尿(D_2)组45例]和70名健康对照者(NC组)的MCP-1基因启动子区A—2518G多态性进行检测,比较各组间基因型频率和等位基因频率。同时检测66例T2DM、70名NC者MCP-1水平。结果 DN(D_1+D_2)组的GA、AA基因型频率和A等位基因频率高于ID_0组(P<0.01)。D_1和ID_2组间基因型频率和等位基因频率无统计学差异。D_0组与NC组间基因型频率有统计学差异,但等位基因频率无统计学差异。GG、GA的人群MCP-1表达水平较AA型者高(P<0.05)。Iogistic回归分析表明MCP-1基因启动子区A—2518G多态性、病程与DN显著相关,GA和AA基因型及病程>5年是DN发生的危险因素。结论壮族人群中MCP-1基因启动子区A 2518G GA、AA基因型可能是DN发生的危险因素;病程>5年可能是DN进展的危险因素。MCP-1启动子区A—2518G基因多态性可影响MCP-1水平。
Objective To investigate the relationship between the polymorphism of MCP-1 promoter region MCP-1 and serum DM-DM and diabetic nephropathy (DN) in Zhuang population. Methods The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to analyze the association between 51 cases of normal albuminuria (D_0), 54 cases of microalbuminuria (D_1) and clinical albuminuria A-2518G polymorphism in the promoter region of MCP-1 gene was detected in 45 patients with D_2) and 70 healthy controls (NC). Genotype frequency and allele frequency were compared between groups. At the same time, 66 cases of T2DM, 70 NC were detected MCP-1 levels. Results The frequencies of GA, AA genotype and A allele in DN (D_1 + D_2) group were higher than those in ID_0 group (P <0.01). There was no significant difference in genotype frequency and allele frequency between D_1 and ID_2 groups. There was a significant difference in genotype frequencies between D_0 group and NC group, but the allele frequencies were not statistically different. The level of MCP-1 in GG, GA was higher than that in AA (P <0.05). Iogistic regression analysis showed that A-2518G polymorphism of MCP-1 gene promoter region, the course of disease was significantly correlated with DN, GA and AA genotypes and course of disease> 5 years were risk factors for DN. Conclusions The genotypes of A 2518G GA and AA in the promoter region of MCP-1 gene in Zhuang population may be risk factors of DN. The duration of> 5 years may be the risk factor of DN progression. MCP-1 promoter region A-2518G gene polymorphism can affect MCP-1 levels.