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目的:探讨阿霉素肾病肾阳虚证病证结合模型的技术方法,为肾脏病的病证结合研究提供技术支撑。方法:40只SD大鼠,按体质量随机分为正常组、阿霉素肾病模型组、阿霉素肾病肾阳虚证模型组、肾阳虚证模型组,分别采用阿霉素、氢化可的松建立阿霉素肾病模型、阿霉素肾病肾阳虚证模型、肾阳虚证模型,并于造模第2、3周末分别测各组体质量、肛温、24h尿蛋白定量、尿-17羟类固醇,第3周末测各组血肌酐、总胆固醇、白蛋白。结果:第2、3周末,各模型组体质量低于正常组(P<0.05,P<0.01),阿霉素肾病模型组、阿霉素肾病肾阳虚证模型组24h尿蛋白定量高于正常组及肾阳虚模型组(P<0.01);第3周末,阿霉素肾病模型组、阿霉素肾病肾阳虚证模型组血白蛋白低于正常组及肾阳虚模型组(P<0.01)、总胆固醇高于正常组及肾阳虚模型组(P<0.01),尿17-羟皮质类固醇含量高于与正常组及肾阳虚模型组(P<0.01)。结论:阿霉素肾病模型的建立是可行的,需进一步评价肾阳虚证模型的建立,为评价阿霉素肾病肾阳虚证结合模型创造条件。
Objective: To explore the technical method of adriamycin nephropathy combined with syndrome of kidney-yang deficiency syndrome and to provide technical support for the combination of disease and syndrome of kidney disease. Methods: Forty SD rats were randomly divided into normal group, adriamycin nephropathy model group, adriamycin-induced nephropathy syndrome model group and kidney-yang deficiency syndrome model group. Adriamycin, Of the pine needles to establish adriamycin nephropathy model, doxorubicin nephropathy kidney yang deficiency model and kidney yang deficiency syndrome model. At the end of the second and third week of modeling, the body weight, rectal temperature, urinary protein excretion in 24 h, -17 hydroxysteroid, the third weekend measured serum creatinine, total cholesterol, albumin. Results: At the 2nd and 3rd weekend, the body weight of each model group was lower than that of the normal group (P <0.05, P <0.01). The urinary protein excretion of 24h group was higher than that of the adriamycin-induced nephropathy model group (P <0.01). At the third weekend, serum albumin in adriamycin-induced nephropathy model group and adriamycin-induced nephropathy model group were lower than those in normal group and kidney-yang deficiency model group (P <0.01) (P <0.01). The total cholesterol was higher than the normal group and the deficiency of kidney - yang model group (P <0.01), and the content of urinary 17 - hydroxycorticosteroid was higher than that of the normal group and the deficiency of kidney - yang model group (P <0.01). Conclusion: The establishment of adriamycin nephropathy model is feasible. It is necessary to further evaluate the establishment of the model of deficiency of kidney-Yang, and to create the conditions for evaluating the combination model of adriamycin-induced nephropathy.