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目的:探讨三氧化二砷(As2O3)、奥沙利铂(L-OHP)协同抑制人肝癌细胞株HepG2细胞增殖及诱导凋亡的作用。方法:采用MTT法检测As2O3、L-OHP单药或联合对HepG2细胞的生长抑制作用;以AnnexinV/PI双染色流式细胞术检测As2O3、L-OHP单药或联合对HepG2细胞凋亡的影响。结果:不同浓度的As2O3、L-OHP单药或联合均可显著抑制HepG2细胞的生长,抑制作用在一定范围内呈浓度依赖性及时间依赖性,两药联合抑制作用明显增强(P<0.05)。0.5,2.0,4.0mg.L-1的As2O3及2.5,5.0mg.L-1的L-OHP所诱导的早期凋亡细胞较对照组明显增多(P<0.01),0.5mg.L-1的As2O3与2.5mg.L-1的L-OHP联合作用可使HepG2细胞早期凋亡细胞明显增多(P<0.01);随着联合浓度增加,晚期凋亡及坏死细胞明显增多(P<0.01)。结论:As2O3及L-OHP均可有效地抑制体外肝癌HepG2细胞的生长并诱导细胞凋亡,两药联合具有协同抑制细胞增殖及诱导凋亡作用。
Objective: To investigate the effects of arsenic trioxide (As2O3) and oxaliplatin (L-OHP) on the proliferation and apoptosis of HepG2 cells. METHODS: MTT assay was used to detect the growth inhibitory effect of As2O3 or L-OHP alone or in combination on HepG2 cells. The effect of As2O3 or L-OHP alone or in combination on the apoptosis of HepG2 cells was detected by Annexin V/PI double staining flow cytometry. . Results: Different concentrations of As2O3, L-OHP alone or in combination can significantly inhibit the growth of HepG2 cells in a dose-dependent and time-dependent manner. The combined inhibition of both drugs was significantly enhanced (P<0.05). . The apoptotic cells induced by 0.5, 2.0, 4.0 mg.L-1 As2O3 and 2.5, 5.0 mg.L-1 L-OHP were significantly increased compared with the control group (P<0.01), 0.5 mg.L-1. The combination of As2O3 and 2.5 mg.L-1 L-OHP significantly increased the number of early apoptotic cells in HepG2 cells (P<0.01). With the increase of combined concentration, late apoptosis and necrotic cells increased significantly (P<0.01). Conclusion: Both As2O3 and L-OHP can effectively inhibit the growth of liver cancer HepG2 cells in vitro and induce apoptosis. The combination of the two drugs has a synergistic effect on inhibiting cell proliferation and inducing apoptosis.