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A·ligosa和另一个野生种亚种的花药于单核花粉粒时期,培养于M·S培养基+2mgNAA+0.5mgBA/1中,获得了愈伤组织。将愈伤组织转移到M·S培
A. ligosa and anther of another wild species subspecies were cultured in MS medium + 2 mg NAA + 0.5 mg BA / 1 during mononuclear pollen stage to obtain callus. Callus is transferred to M · S culture