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1963年Piper等报告10例胃癌患者胃液β-葡萄糖醛酸酶活力全部升高,继而Kim,Harada等均肯定胃液内此酶活力测定对胃癌诊断的价值。从74年10月以来,我院采用与Kim等相似的方法,测定152例胃液β-葡萄糖醛酸酶活力,结果报告如下: 测定方法 一、试剂 1.0.01M酚酞葡萄糖醛酸基质:称0.788克酚酞葡萄糖醛酸辛可尼丁碱纯品(按文献方法合成),放于烧杯中加60ml蒸馏水及20ml 2N盐酸,搅拌使其溶解。将溶液移入分液漏
In 1963, Piper et al. reported that the activity of β-glucuronidase in gastric juice was increased in 10 patients with gastric cancer. Kim, Harada et al. confirmed the value of this enzyme activity measurement in gastric juice for the diagnosis of gastric cancer. Since October, 74, our hospital has used the method similar to Kim et al. to determine the activity of β-glucuronidase in 152 cases of gastric juice. The results are reported as follows: Method 1. Reagents 1.0.01M phenolphthalein glucuronic acid matrix: 0.788g Pure cinnamic acid phenolphthalein glucuronide (synthesized by literature method) was placed in a beaker and 60 ml of distilled water and 20 ml of 2N hydrochloric acid were added and stirred to dissolve. Move the solution into the liquid leak