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目的 探讨低温海水淹溺大鼠心肌ＴＸＢ２、６－Ｋｅｔｏ－ＰＧＦ１α及ＴＸＢ２／６－Ｋｅｔｏ－ＰＧＦ１α（Ｔ／Ｋ）与血气的关系。方法 将淹溺组大鼠置于低温（１６～１８℃）海水中，任其自由游动直至沉入水底，呼吸暂停，迅速取出置室温下观察，分别于５，１５，３０，６０，２４０，３６０ ｍｉｎ（每小组各６只）测大鼠肛温，取左心血行血气分析，取各小组４只检测心肌组织ＴＸＢ２及６－Ｋｅｔｏ－ＰＧＦ１α含量，观察Ｔ／Ｋ变化，肺组织计算干重／湿重比值（Ｄ／Ｗ），另２只光镜下观察肺脏及心脏形态改变。结果 淹溺大鼠肛温、ｐＨ、ＰａＯ２及 ６－Ｋｅｔｏ－ＰＧＦ１α明显降低，于５ｍｉｎ组降至最低点（Ｐ＜０．０１），ＰａＣＯ２及 ＴＸＢ２则明显上升，分别于５ｍｉｎ组及３０ｍｉｎ组升至最高点（Ｐ＜０．０１），Ｔ／Ｋ比值明显上升；淹溺后随离海水的时间延长，肛温、ｐＨ、ＰａＯ２及Ｐａ－ＣＯ２呈恢复趋势，心肌的ＴＸＢ２、６－Ｋｅｔｏ－ＰＧＦ１α及Ｔ／Ｋ比值则无明显改善。结论 低温海水淹溺大鼠心肌的ＴＸＢ２及６－Ｋｅｔｏ－ＰＧＦ１α含量受低温、低氧血症及酸中毒的影响，造成ＴＸＢ２增高、６－Ｋｅｔｏ－ＰＧＦ１α降低及Ｔ／Ｋ比值失衡从而使心脏功能趋于恶化。
Objective To investigate the relationship between TXB2, 6-Keto-PGF1α and TXB2 / 6-Keto-PGF1α (T / K) and blood gas in the myocardium of rats drowning in seawater. Methods The rats in the drowning group were placed in seawater at low temperature (16-18 ℃), allowed to swim freely until they were submerged, suspended in air and quickly taken out for observation at room temperature. The rats were sacrificed at 5, 15, 30, 60, 240 , 360 min (6 rats in each group). The rectal temperature was measured in left anterior descending artery and the left ventricular blood was collected for blood gas analysis. The content of TXB2 and 6-Keto-PGF1α in myocardium of each group were measured. The changes of T / K, Weight / wet weight ratio (D / W), the other two light microscope observation of lung and heart shape changes. Results The rectal temperature, pH, PaO2 and 6-Keto-PGF1α in the rats were significantly lower than those in the 5min group (P <0.01), PaCO2 and TXB2 were significantly increased in the 5min group and 30min group (P <0.01), and the T / K ratio increased significantly. After prolonged exposure to seawater, the rectal temperature, pH, PaO2 and Pa-CO2 showed a trend of recovery. The levels of TXB2, 6-Keto- PGF1α and T / K ratio was no significant improvement. Conclusion The levels of TXB2 and 6-Keto-PGF1α in myocardium of rats drowning in low temperature seawater are affected by hypothermia, hypoxemia and acidosis, resulting in the increase of TXB2, the decrease of 6-Keto-PGF1α and the imbalance of T / K ratio, Tends to deteriorate.