干扰素作为一种有效的药物已用于慢性丙型肝炎的治疗,但对停用干扰素后肝炎复发的机制尚不清楚,尤其是已表明血清HCVRNA的消失并不预示对干扰素有长期反应。为了评价干扰素对HCV复制的疗效,作者利用一种选择性、半定量逆转录PCR(RT-PCR)方法,检测用干扰素治疗的慢性丙型肝炎患者肝、外周血单核细胞(PBMCs)和血清中的HCV RNA正、负链。 Interferon, as an effective drug, has been used in the treatment of chronic hepatitis C, but the mechanism of hepatitis relapse after discontinuation of interferon is unclear. In particular, it has been shown that the disappearance of serum HCV RNA does not predict long-term response to interferon . To evaluate the efficacy of interferon in HCV replication, we used a selective, semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) method to detect hepatic and peripheral blood mononuclear cells (PBMCs) in patients with chronic hepatitis C treated with interferon And positive and negative HCV RNA in serum.