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本研究将脑内在体（invivo）微透析采样技术和高效液相色谱-荧光检测分析法相结合，监测了猕猴（Macacamulatta）在履行一种延缓go／no-go任务中其屋核内的兴奋性氨基酸水平的变化。在这种行为任务操作中，猴必须根据记忆中保持的暗示信号的位置来发动（go）或抑制（no－go）随后的运动反应，因此具有短时工作记忆性质。我们发现，在这种行为任务操作过程中，猴尾核透析液中的Glu和Asp水平较操作前基础水平分别降低（31．68±3．85）％（n=10）和（26.25±5.95）％（n=10），具有极显著的统计意义（Glu：t9=6．51，P〈0．001；Asp：t9=3．39，P<0．01）。同时，透析液中的Gln和Asn水平也显著降低（P<0．05）。相反，在没有延缓期的go／no－go任务和单纯的延缓go任务操作过程中，尾核透析液中Glu，Asp，Gln和Asn的水平均无显著性变化。这一结果提示，尾核内的兴奋性氨基酸递质传递参与延缓go/no-go任务的操作过程，为尾核内谷氨酸能传递参与运动工作记忆调控提供了直接的实验证据。
In this study, the combination of microdialysis in vivo (invivo) microdialysis technique and high performance liquid chromatography-fluorescence detection assay was used to monitor the excitability of Macaca mulatta in performing a delayed go / no-go task Changes in amino acid levels. In this behavioral task operation, the monkey must either go or suppress subsequent motional responses based on the position of the implied signal held in memory and therefore have a short-term working memory property. We found that the levels of Glu and Asp in monkey caudate nucleus were decreased by 31.68 ± 3.85% (n = 10) and (26.25 ± 5.95), respectively, compared with pre-operation basal levels during this behavioral task. )% (N = 10), with extremely significant statistical significance (Glu: t9 = 6.51, P <0.001; Asp: t9 = 3.39, P <0.01). At the same time, the levels of Gln and Asn in dialysate were also significantly reduced (P <0.05). In contrast, there was no significant change in the levels of Glu, Asp, Gln and Asn in the caudate nuclei of dialysis fluid during the go / no-go task and the simple go task without delay. This result suggests that excitatory amino acid neurotransmitters in the caudate nucleus participate in delaying the operation of go / no-go tasks and provide direct experimental evidence for the involvement of glutamate energy transmitter in the caudate nucleus.