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目的探讨水飞蓟宾对高脂诱导的非酒精性脂肪肝(NAFL)模型大鼠的调脂保肝作用。方法采用ig高脂乳剂配合高脂饲料制备大鼠NAFL模型,持续4周,对照组给予生理盐水和普通饲料。模型大鼠随机分为模型组、辛伐他汀(阳性药,1.8 mg/kg)组和水飞蓟宾低、中、高剂量(18.9、37.8、75.6 mg/kg)组,第5周在继续造模的基础上ig给药,每天1次,持续8周。末次给药后,称取肝脏质量并计算肝系数;HE染色观察肝组织病理形态;腹主动脉取血,分离血清,试剂盒法检测三酰甘油(TG)、总胆固醇(TC)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)、天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)水平。结果连续治疗8周后,与对照组比较,水飞蓟宾高、中剂量均可明显改善肝组织脂肪变性程度;各剂量组肝系数均显著下降(P<0.05、0.01);各剂量组均显著降低血清TC、TG、AST、ALT水平(P<0.05、0.01);高、中剂量组显著降低LDL、升高HDL水平(P<0.01)。结论水飞蓟宾对NAFL大鼠发挥治疗作用,其作用可能与降脂、保肝有关。
Objective To investigate the effect of silybin on the lipid-lowering and hepatoprotective effect of high fat-induced non-alcoholic fatty liver disease (NAFL) model rats. Methods NAFL model was made by using ig high fat emulsion and high fat diet for 4 weeks. The control group was given normal saline and normal feed. The model rats were randomly divided into model group, simvastatin (1.8 mg / kg) group and silybin low, medium and high doses (18.9, 37.8, 75.6 mg / kg) On the basis of modeling ig administration, 1 day, for 8 weeks. After the last administration, the liver mass was weighed and the liver coefficient was calculated; the histopathology of liver tissue was observed by HE staining; the blood was taken from the abdominal aorta and the serum was separated; the triglyceride (TG), total cholesterol (TC) Lipoprotein (HDL), low density lipoprotein (LDL), aspartate aminotransferase (AST), alanine aminotransferase (ALT) levels. Results After continuous treatment for 8 weeks, compared with the control group, the silybin high and medium doses could significantly improve the degree of hepatic steatosis; the liver coefficient of each dose group was significantly decreased (P <0.05, 0.01); each dose group The levels of TC, TG, AST and ALT in serum were significantly decreased (P <0.05, 0.01). The levels of LDL and HDL in high and middle dose groups were significantly decreased (P <0.01). Conclusion Silybin on NAFL rats play a therapeutic role, its role may be related to lipid-lowering, liver protection.