论文部分内容阅读
目的比较人视网膜祖细胞和脑神经干细胞的体外分化潜能。方法分离8～12周人胎儿神经视网膜和脑皮质、纹状体神经干细胞,进行无血清体外培养;采用光镜和免疫细胞化学或荧光免疫细胞化学染色方法,分别观察在无血清和10%胎牛血清培养条件下,两种来源的神经干细胞分化后的细胞特性。结果两种来源的神经干细胞,均能在体外有或无血清培养条件下增殖并分化。视网膜祖细胞不但表达视网膜祖细胞标志物 Pax-6,也可表达神经干细胞标志物-巢蛋白(Nestin)、成熟神经元标志物-微管相关蛋白2(Map2)、星形胶质细胞标志物-胶质纤维酸性蛋白(GFAP)、节细胞标志物 Thy-1和视杆细胞标志物视紫红质(Rhodopsin);脑神经干细胞也能表达这些特异性细胞标志物。血清诱导分化时,视网膜祖细胞较难贴壁,贴壁细胞球伸出少而短的突起,单个细胞形态不清;而脑神经干细胞球易贴壁并伸出较长突起交织成网,大量细胞从细胞球中沿突起徙出,单个细胞形态清晰。结论人胎儿视网膜祖细胞和脑神经干细胞体外培养均具有向神经元、胶质细胞及视网膜终末细胞分化的能力;两种干细胞在进行血清诱导分化时,细胞的贴壁、迁移能力及分化后的细胞形态均存在差异。(中华眼科杂志,2006,42:901-907)
Objective To compare the differentiation potential of human retinal progenitor cells and neural stem cells in vitro. Methods Human fetal neural retina and cerebral cortex and striatum neural stem cells were isolated from 8 to 12 weeks and cultured in serum-free medium. Using light microscopy and immunocytochemistry or fluorescent immunocytochemical staining, Bovine Serum Cultured Cells After Differentiation of Two Types of Neural Stem Cells. Results Both types of neural stem cells proliferated and differentiated in vitro or in serum-free culture. Retinal progenitor cells not only express Pax-6, a marker of retinal progenitor cells, but also express neural stem cell marker Nestin, mature neuronal marker-microtubule-associated protein 2 (Map2), astrocyte marker - glial fibrillary acidic protein (GFAP), the cell marker Thy-1 and the rod cell marker Rhodopsin; brain neural stem cells can also express these specific cell markers. Serum induced differentiation, retinal progenitor cells more difficult to adherent, adherent cells protruding a small number of short and short protrusions, single cell morphology is not clear; and neural stem cell spheres easy to stick to the wall and stick out a long process of interwoven into a large number Cells migrate along the protuberances from the cytoplasm, and the shape of individual cells is clear. Conclusion Human fetal retinal progenitor cells and neural stem cells cultured in vitro have the ability to differentiate into neurons, glial cells and retinal terminal cells. When the two kinds of stem cells are differentiated into cells in vitro, the adhesion, migration and differentiation of the cells There are differences in cell morphology. (Chinese Journal of Ophthalmology, 2006,42: 901-907)