论文部分内容阅读
目的探讨替米沙坦阻断肾素-血管紧张素系统(RAS)对db/db小鼠胰岛分泌功能的影响。方法将8周龄db/db小鼠随机分为两组,分别给予6周替米沙坦或安慰剂灌胃治疗,同周龄的db/m小鼠作为非糖尿病对照。喂药6周后行糖耐量实验,然后取胰腺,进行胰岛素免疫组化检测,另用离体胰岛灌流系统测定胰岛素第一时相的分泌。结果替米沙坦组治疗6周后空腹血糖显著下降(P<0.05)。糖负荷120 min后葡萄糖曲线下面积(AUC)低于安慰剂对照组(P<0.05),AUC_(Ins0～30)高于安慰剂对照组(P<0.05)。离体胰岛灌流结果显示,替米沙坦组胰岛素第一时相分泌得到改善。胰岛内胰岛素表达强度替米沙坦组明显高于安慰剂对照组。结论替米沙坦能改善糖耐量,恢复胰岛素第一时相分泌,保护β细胞功能。
Objective To investigate the effect of telmisartan blocking renin-angiotensin system (RAS) on insulin secretion in db / db mice. Methods 8-week-old db / db mice were randomly divided into two groups, treated with telmisartan or placebo for 6 weeks, and db / m mice of the same age as non-diabetic controls. Glucose tolerance test was performed 6 weeks after the drug was given, then the pancreas was taken for insulin immunohistochemistry, and the secretion of the first phase of insulin was measured by the isolated islet perfusion system. Results Fasting blood glucose decreased significantly (P <0.05) 6 weeks after treatment in the telmisartan group. The area under the curve of glucose (AUC) after 120 min of glucose loading was lower than that of the placebo control group (P <0.05), and the AUC_ (Ins0 ~ 30) was higher than that of the placebo control group (P <0.05). In vitro perfusion results showed that telmisartan group first phase insulin secretion improved. Insulin expression intensity in islets was significantly higher in the telmisartan group than in the placebo control group. Conclusion Telmisartan can improve glucose tolerance, restore the first phase of insulin secretion and protect the function of β cells.