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目的评价舒芬太尼对小鼠单侧坐骨神经损伤后再生的影响。方法选取健康Balb/c小鼠160只,制作单侧坐骨神经离断损伤模型。采用随机数字表法分为4组,即舒芬太尼高(H)、中(M)、低(L)剂量组和对照(B)组,H组腹腔注射舒芬太尼100μg/(kg·d),M组50μg/(kg·d),L组25μg/(kg·d),B组腹腔注射0.2 ml生理盐水。连续给药7 d。每组小鼠分别在给药后1、3、5 d和1、2、4、8、12周8个时间点进行神经电生理测试,每个时间点每个剂量组5只小鼠。检测坐骨神经再生后神经的传导波幅及传导速度,再处死小鼠,取其坐骨神经损伤处上下0.5 cm的神经组织进行镀银染色,观察神经纤维的再生形态及免疫组化检测S-100蛋白的表达。结果 H组给药4、8、12周动作电位波幅分别为(5.13±0.20)、(24.36±0.13)、(26.17±0.44)mv,M组分别为(4.76±0.19)、(19.19±0.09)、(23.71±0.09)mv,与B组同时点[(1.99±0.18)、(13.78±0.11)、(17.90±0.66)mv]比较,差异均有统计学意义(P<0.05)。H组给药4、8、12周神经传导速度分别为(40.1±0.7)、(60.9±1.2)、(66.1±0.8)m/s,M组分别为(33.5±0.7)、(55.6±1.1)、(60.2±0.7)m/s,与B组同时点[(25.6±0.8)、(50.2±0.3)、(50.7±1.1)m/s]比较,差异均有统计学意义(P<0.05)。给药后8周镀银染色显示,H组的神经纤维排列较为整齐,很少有溶解现象;M组稍差,排列较整齐,有溶解现象;L组和B组的排列紊乱,多数神经纤维溶解。各剂量组损伤后S-100蛋白表达逐渐增加,到2周时达到高峰,然后逐渐下降。与B组比较,各时间点H、M组的S-100的表达均显著增加(P<0.05)。结论舒芬太尼可以通过促进S-100的表达,促进周围神经损伤后的再生。
Objective To evaluate the effect of sufentanil on the regeneration of unilateral sciatic nerve in mice. Methods 160 healthy Balb / c mice were selected to make unilateral sciatic nerve injury model. The patients were randomly divided into 4 groups: sufentanil (H), middle (M), low (L) and control group (B) · D), M group 50μg / (kg · d), L group 25μg / (kg · d), B group intraperitoneal injection of 0.2 ml saline. Continuous administration of 7 d. Each group of mice were subjected to electrophysiological electrophysiological tests at 1, 3, 5, and 1, 2, 4, 8 and 12 weeks after administration, respectively, and 5 mice in each dose group at each time point. The nerve conduction amplitude and conduction velocity were measured after regeneration of sciatic nerve. The mice were sacrificed and the nerve tissue of 0.5 cm above the sciatic nerve injury was plated with silver staining to observe the regenerated morphology of nerve fiber and the expression of S-100 protein by immunohistochemistry . Results The amplitude of action potentials at 4, 8 and 12 weeks in group H were (5.13 ± 0.20), (24.36 ± 0.13) and (26.17 ± 0.44) mv, respectively, and those in group M were (4.76 ± 0.19) and (19.19 ± 0.09) , (23.71 ± 0.09) mv, respectively, which were significantly different from those in group B (1.99 ± 0.18, 13.78 ± 0.11, and 17.90 ± 0.66 mv respectively) (P <0.05). The nerve conduction velocity at 4, 8 and 12 weeks in H group were (40.1 ± 0.7) and (60.9 ± 1.2) and (66.1 ± 0.8) m / s, respectively, and those in M group were 33.5 ± 0.7 and 55.6 ± 1.1 ), (60.2 ± 0.7) m / s, respectively, which were significantly different from those in group B at the same time point (25.6 ± 0.8, 50.2 ± 0.3, 50.7 ± 1.1) m / ). Eight weeks after the administration, silver staining showed that the nerve fibers in group H were arranged neatly with few dissolution phenomena; group M was slightly worse and arranged neatly with lysis; the arrangement of group L and group B was disordered. Most nerve fibers Dissolved. The expression of S-100 protein in each dose group increased gradually and peaked at 2 weeks, then decreased gradually. Compared with group B, the expression of S-100 in H and M groups at each time point increased significantly (P <0.05). Conclusion Sufentanil can promote the regeneration of peripheral nerve injury by promoting the expression of S-100.