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目的 研究基质金属蛋白酶MMP 2、9和组织抑制因子TIMP 1、2在子宫内膜异位症中的表达及意义。方法 2 0 0 0年 3月至 2 0 0 2年 4月应用免疫组化S P法检测卵巢巧克力囊肿 4 5例的异位内膜和其中 2 0例在位内膜 ,以及 2 2例正常子宫内膜中MMP 2、9及TIMP 1、2的表达。结果 异位内膜组织中MMP 2、9和TIMP 1、2的表达均显著高于在位内膜和正常内膜 (P <0 0 1)。在位内膜和正常子宫内膜组织细胞中的表达率比较 ,差异无显著性 (P >0 0 5 )。子宫内膜异位症组织中MMP 2、9的表达与侵袭程度正相关。TIMP 1、2的表达则与侵袭程度呈负相关。结论 MMP 2、9是检测子宫内膜异位症较好的分子标志 ,人工诱导TIMP 1、2或阻断MMP 2、9的表达可能抑制内异症的发生发展 ,有望成为子宫内膜异位症治疗新的辅助手段
Objective To study the expression and significance of matrix metalloproteinase (MMP 2,9) and tissue inhibitor of metalloproteinase (TIMP 1,2) in endometriosis. Methods From March 2000 to April 2002, immunohistochemical SP method was used to detect the ectopic endometrium of 45 cases of ovarian chocolate cysts and 20 cases of eutopic endometrium, as well as 22 normal uterus Endometrial MMP 2, 9 and TIMP 1,2 expression. Results The expression of MMP 2, 9 and TIMP-1 in ectopic endometrium was significantly higher than that in eutopic endometrium and normal endometrium (P <0.01). There was no significant difference in the expression rates of eutopic endometrium and normal endometrium (P> 0.05). The expression of MMP 2,9 in endometriosis was positively correlated with the degree of invasion. TIMP 1,2 expression was negatively correlated with the degree of invasion. Conclusions MMP 2,9 is a good molecular marker for detection of endometriosis. Artificially induced expression of TIMP 1,2 or MMP 2,9 may inhibit the development of endometriosis and is expected to become endometriosis Symptoms treatment of new aids